Journal: Frontiers in Molecular Biosciences
Article Title: Arsenic trioxide-induced acute kidney injury: OPA1- and Drp1-mediated mitochondrial dynamics imbalance, PINK1/Parkin-dependent mitophagy, and Chuanhuang Fang III
doi: 10.3389/fmolb.2026.1778855
Figure Lengend Snippet: CHF protects against AI-AKI by modulating renal tubular epithelial cell MQC in rats. (A, B) Mitochondrial membrane potential (Δψm) was detected by JC-1 staining. (C–F) OPA1 and Drp1 expression was assessed by immunofluorescence (scale bar = 40 µm) in rat kidney tissues. (G, H) Flow cytometry results of mitochondria ROS in rat kidney tissues. (I) Western blotting analysis of OPA1, Drp1, BNIP3, NIX, Parkin, PINK1, LC3B, Bcl-2 and BAX. (p <0.05, *p <0.01 vs CON or ATO or NAC, n=3).
Article Snippet: Human renal proximal tubular epithelial cells (HK-2, American Type Culture Collection, Manassas, VA, United States) were cultured in DMEM/F12 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 .
Techniques: Membrane, Staining, Expressing, Immunofluorescence, Flow Cytometry, Western Blot